Stable isotopes of hydrogen and oxygen are often used for water balance calculations of lakes. We present an approach combining the lake water balance with an isotope mass balance to constrain the sources and sinks of the water of a small dimictic lake subjected to eutrophication. Meteorological and hydraulic data in combination with measured isotope signatures of the different water compartments enabled to assess the degree of surface water/groundwater interaction and the amount of overland flow into the lake. Groundwater could be excluded as a lake water source, as its water level was always below the lake water level. In the absence of a channelled inflow, precipitation and overland flow were the remaining options, whereby the latter was only active during periods of exceptionally high rainfall. While the groundwater signatures adjacent to the lake showed an influence of lake water, the lake water balance itself indicated that the associated volumetric water loss to groundwater is rather negligible. In the present case, only a combined assessment of hydrological and isotopic data allowed for an accurate characterization of the studied lake and a quantification of its water sources and sinks, highlighting the importance of using more than one methodological approach for such a purpose.