Increasing fish meal prices and the depletion of natural fish stock due to fish meal production demands for novel feed ingredients in aquaculture. Marine amphipods are a natural food source for many flatfish species and are rich in essential fatty acids hence strong candidates as fish meal replacement in aquafeeds. Recent studies showed promising fatty acid profiles and fatty acid synthesis in marine amphipods, which could lower the need for fish oil supplementation in finfish feeds. In this study, juvenile turbot Psetta maxima were fed with four different diets containing different levels of amphipod meal as fish meal replacement: 0, 50 and 100 % of replacement and commercial turbot feed as reference. The experimental diets were formulated regarding basic nutritional demands without fish oil added. Growth performance as well as lipid classes and fatty acid profile in muscle and liver tissue in response to different feeding regimes were investigated.