Lake Sedimentary DNA Research on Past Terrestrial and Aquatic Biodiversity: Overview and Recommendations


Contact
ulrike.herzschuh [ at ] awi.de

Abstract

The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises



Item Type
Article
Authors
Divisions
Primary Division
Programs
Primary Topic
Helmholtz Cross Cutting Activity (2021-2027)
N/A
Publication Status
Published
Eprint ID
54737
DOI 10.3390/quat4010006

Cite as
Capo, E. , Giguet-Covex, C. , Rouillard, A. , Nota, K. , Heintzman, P. D. , Vuillemin, A. , Ariztegui, D. , Arnaud, F. , Belle, S. , Bertilsson, S. , Bigler, C. , Bindler, R. , Brown, A. G. , Clarke, C. L. , Crump, S. E. , Debroas, D. , Englund, G. , Ficetola, G. F. , Garner, R. E. , Gauthier, J. , Gregory-Eaves, I. , Heinecke, L. , Herzschuh, U. , Ibrahim, A. , Kisand, V. , Kjær, K. H. , Lammers, Y. , Littlefair, J. , Messager, E. , Monchamp, M. E. , Olajos, F. , Orsi, W. , Pedersen, M. W. , Rijal, D. P. , Rydberg, J. , Spanbauer, T. , Stoof-Leichsenring, K. R. , Taberlet, P. , Talas, L. , Thomas, C. , Walsh, D. A. , Wang, Y. , Willerslev, E. , van Woerkom, A. , Zimmermann, H. H. , Coolen, M. J. , Epp, L. S. , Domaizon, I. , G. Alsos, I. and Parducci, L. (2021): Lake Sedimentary DNA Research on Past Terrestrial and Aquatic Biodiversity: Overview and Recommendations , Quaternary, 4 (1), p. 6 . doi: 10.3390/quat4010006


Share
Add to AnyAdd to TwitterAdd to FacebookAdd to LinkedinAdd to PinterestAdd to Email


Citation

Geographical region

Research Platforms

Campaigns
N/A

Funded by
info:eu-repo/grantAgreement/EC/H2020/772852


Actions
Edit Item Edit Item